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 首頁 > 產品展示 > 安捷倫液相色譜儀 > 液相色譜儀 > ELSD 安捷倫G4218
 
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Waters 2424揮發性光散射偵測器(ELSD)
 
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產品型號:
安捷倫380
產品名稱:
ELSD 安捷倫G4218
產品報價:
0
  安捷倫380ELSD 安捷倫G4218的詳細資料:

 Agilent 380 ELSD  G4218





 

Introduction to the ELSD(簡介)

  The Evaporative Light Scattering Detector (Agilent 380/385-ELSD) is a unique

and highly sensitive detector for semi-volatile and non-volatile solutes in a

liquid stream. It is mainly used as a concentration detector for High

Performance Liquid Chromatography (HPLC). The solvent stream containing

the solute material is nebulized and carried by a gas flow through an

evaporation chamber. The solvent is volatilized, leaving a mist of solute

particles that scatter light to a photosensitive device. The signal is amplified

and a voltage output provides the concentration of the solute particles passing

through the light.

The Agilent 380/385-ELSD may be used alone, or as one of several detectors in

a GPC or HPLC system. As the solvent or eluent is evaporated in the course of

the analysis, the Agilent 380/385-ELSD must be the last in series if used in

conjunction with other detectors. If the Agilent 380/385-ELSD is being used as

the last detector in a series, care must be taken not to exceed the

recommended backpressure in detector cells in other units.

This manual instructs the user in the installation and operation of the Agilent

380/385-ELSD for stand-alone use and control using Agilent OpenLAB

(ChemStation & EZChrom editions) and Agilent ChemStation.

 

 

Operational Parameters(操作參數)

 

  The ELS Detector responds to all compounds that are less volatile than the

mobile phase and is independent of a compound’s optical properties. It

therefore provides advantages over other spectroscopic detectors for detecting

compounds that are deficient in a UV chromophore or fluorophore.

The removal of aqueous mobile phase within an ELSD is typically achieved by

setting the evaporator temperature to the eluent’s boiling point (e.g. 100 °C) in

order to remove the solvent. For non-volatile compounds, operating at these

high temperatures maximizes the signal response.

However, at these temperatures volatile and semi-volatile compounds are

destroyed and are therefore not detected. This is problematic for small

molecules, such as pharmaceuticals and drug candidates.

The ELS detectors are specifically designed to evaporate difficult solvents at

ambient and sub-temperatures in order to maximize detection of semi-volatile

compounds.

The ELSD has patented technology that reduces the evaporation time of highly

aqueous solvents at low temperature, and also prevents the evaporation tube

becoming saturated, which would otherwise preventing further evaporation

occurring.

Using this patented evaporation gas technology, a 20 μm droplet of water at

30 °C can be dried ca. 3x faster than just temperature alone. Using the Agilent

385-ELSD (G4261A), water can be evaporated as low as 20 °C, providing

maximum sensitivity to thermally sensitive compounds. For maximum

sensitivity of non-volatile compounds the evaporation gas can be turned off at

higher evaporation temperatures.

Therefore, unlike other ELS detectors, where the evaporator temperature is

set according to the type of mobile phase, the ELS detector evaporator

temperature is independent of the mobile phase. Consequently, the ELS

detector can be set at 30 °C for all types of mobile phase provided the

evaporator gas flow is adjusted accordingly. This method of operation ensures

that the ELSD sensitivity is maximized even for low molecular weight

compounds.

  To prevent against unnecessary gas usage, a controlled gas shut off valve is

integrated into the detector gas manifold. This will only allow gas to pass into

the instrument when in RUN mode. Should the instrument default to

STANDBY mode the gas will reduce to a default value of 1.2 SLM for 15 min

before closing.

 Parts and Materials(零件與材料)

Evaporative Light Scattering Detector Parts 蒸發光散射檢測器部分

 



序號

說明

部件號

1

Nebulizer Assembly [1]

see list below

2

Nebulization Chamber(glass)[2]

G4218-40000

3

Black Plastic Nut for Nebulization Chamber (13 mm Diameter) [3]

G4218-40010

9

Black Plastic Nut for Nebulization Chamber (30 mm Diameter) [9]

G4218-40011

Seal kit for nebulization chambe

G4218-68010

Cartridge for Gas Regulator

G4218-40150

4、5

Drain Assembly (includes fitting [5]) [4]

G4218-40100

Drain Bottle Assy

G1946-60111

¾ inch PE Tubing for Drain Bottle, 1 unit = 1 cm

0890-1727

Tube Fitting (6 mm diameter) for Gas Regulator

G4218-40160

6

Pneumatic tube (diameter 4 mm) for Nebulizer

(includes stainless steel fitting [6])

G4218-40220

Detector Gas Inlet Tube 6 mm O.D., 10 m

G4218-40170

7

Wall Mounting Fitting (4 mm diameter) [7]

G4218-40140

8

Bulkhead Fitting [8]

G4218-40130

11

Gas check valve

G4218-40200

Front panel shield window

G4218-40400

Autozero Cable

G4218-81101

Signal Cable

G4218-81100

RS232 Cable Female/Female/Straight, 3 m

G4218-81103

External Event Cable

G4218-81102

APG Remote Cable

5061-3378

Caffeine Standard 250 μg/ml in water

G4218-85000

 

 

Nebulizers for the G4218A Agilent Evaporative Light Scattering Detector

(用于G4218A霧化器)

Nebulizer

Useable Flow

Range

Optimum

Flow Range

Nebulizer Back

Pressure - bar

(with water)

Identifying

Marks

Part Number

Micro Flow

Nebulizer

5.0 μl/min –

40.0 μl/min

15.0 μl/min –

25.0 μl/min

24 (100 μl/min

Blue Seal

2 Rings

G4218-20003

Semi Micro Flow

Nebulizer

0.04 ml/min –

1.0 ml/min

0.1 ml/min –

0.3 ml/min

44 (1 ml/min)

Yellow Seal

2 Rings

G4218-20001

Standard Flow

Nebulizer

0.2 ml/min –

2.0 ml/min

0.5 ml/min –

1.2 ml/min

4 (1 ml/min)

Black Seal

2 Rings

G4218-20000

Large Flow

Nebulizer

1.0 ml/min –

5.0 ml/min

2.0 ml/min –

3.0 ml/min

4 (1 ml/min)

Red Seal

1 Ring

G4218-20002

Rapid Resolution

Nebulizer

0.2 ml/min –

1.4 ml/min

0.4 ml/min –

1.0 ml/min

14†

White Seal

1 Ring

G4218-20004

 

 

Identifying Parts and Materials(識別零件與耗材)

p/n

Description

G4260A

Agilent 380 Evaporative Light Scattering Detector

G4261A

Agilent 385 Evaporative Light Scattering Detector

G4260-60005

Injection Trigger Cable

PL0890-0300

Analogue output cable

PL0890-0305

Gas inlet tube (2 m)

PL0890-0310

Exhaust hose (PVC-2 m)

PL0890-0315

Waste hose (tygon) 7 cm

PL0890-0320

Solvent waste container (500 mL)

PL0890-0325

RS232 communication cable

PL0890-0345

Dimension trigger cable

PL0890-0350

Remote start cable (third party LCs only)

G4260-90000

ELSD Operation Manual

PL0890-0370

ELSD Control Software

PL0890-0375

ELSD Dimension Software (includes trigger cable)

PL0890-0390

ELSD nebuliser

PL0890-0395

Leak sensor assembly

PL0890-0400

Mass flow controller

PL0890-0405

Photo detector assembly

PL0890-0410

ELSD light source

PL0890-0415

Fan assembly

PL0890-0420

Front (white) LED assembly

PL0890-0425

Gas inlet assembly

PL0890-0430

AR20 regulator assembly

PL0890-0435

Front outlet tube

PL0890-0440

ELSD key

PL0890-0445

Front panel moulding

PL0890-0450

Solvent inlet connector (1/16th)

PL0890-0455

ELSD grey keypad

PL0890-0460

380-ELSD display window

PL0890-0465

385-ELSD display window

PL0890-0470

380-ELSD main control PCB

PL0890-0475

Evaporator cartridge

PL0890-0480

Evaporator heater assembly (no cartridge)

PL0890-0485

Neb mount assembly

PL0890-0490

Neb chamber assembly

PL0890-0495

Exhaust tube

PL0890-0500

Exhaust waste tube

PL0890-0505

Glass sleeve, neb chamber

PL0890-0510

Vapour sensor

PL0890-0515

385-ELSD main control PCB

PL0890-0520

380-ELSD power supply

PL0890-0525

Gas inlet frit, 10 μm

PL0890-0530

Gas inlet plug

PL0890-0535

385-ELSD power supply

PL0890-0540

FFKM O-ring (19.30x2.40)

PL0890-0545

FFKM Neb O-Ring (6.0x1.0)

PL0890-0550

PTFE nebuliser chamber

PL0890-0555

Nebuliser waste tube

PL0890-0560

P-Trap diffusor

PL0890-0565

Viton O-ring (19.30x2.40)

PL0890-0570

Viton O-ring (0.7x1.0)

PL0890-0575

Viton O-ring (15.30x2.40)

PL0890-0580

Light trap assembly

PL0890-0585

Prism assembly

PL0890-0595

Optics heater

PL0890-0615

O-ring (21.95x1.78)

PL0890-0620

O-ring nitrile, prism

PL0890-0630

385-ELSD peltier assembly

PL0890-0635

Mains inlet fuse (Pk 5)

8121-1013

Serial/USB adapter

 

 

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